The Effect of the Calcium Antagonist and Steroid on the Cat Model of Experimental Cerebral Ischemia.

Shin, Kyu Man; Kim, Sung Hak

Original Article

Journal of Korean Neurosurgical Society 1989;18(2): 226-234.

Department of Neurosurgery, College of Medicine, Ewha Womans University, Seoul, Korea.

ABSTRACT

The purpose of this study to determine the therapeutic effect of nimodipine(N.P.) and methylprednisone(M.P.) on the changes of cytochrome oxidase activity(COA) and lipid peroxidation(L.P.) in the acute phase of focal cerebral zschenia. Acute focal cerebral ischemia was induced by transorbital occlusion of proximal part of the left middle cerebral artery(MCA) with Heifetz clip under the operating microscope. The experimental animals were devided into 6 groups. The occlusion group was that the acute focal ischemia was induced by 4-hour of occlusion of the MCA only. The circulation group was that of 2-hour recirculation after acute focal ischemia. The steroid treatment group was that of M.P.(15 mg/kg) initial intravenous injection at 30 minutes after occlusion and then repeated injection at intervals of one half hours. The N.P. treatment group was that of continuous intravenous N.P.(1 microkilogram/min) infusion, initiated 30 minutes after induction of the ischemia. The combined treatment group was that was treated with intravenous infusions of M.P. as the same procedures with the M.P. and N.P. treatment groups. The sham control group was the animals with removal of the orbital contents with no occlusion of MCA. The results obtained were as follows : In the steroid treatment group COA was significantly increased to 189.76% and L.P. significantly reduced to 53.14% and the N.P. treatment groups COA was significantly increased to 170.88% and L.P. significantly reduced to 56.20% of the values of the occlusion group, respectively. In the combined treatment group COA was very significantly 220.35% and L.P. reduced highly significantly decreased to 48.85% of the values of the occlusion group. It can be concluded the above results indicate the therapy of N.P. combination with M.P. has the therapeutic effect in the management of acute focal ischemia of this cat model.

Key Words: Ischemia nimodipine; Methylprednisolone; Cytochrome oxidase; Lipid peroxidation