| The Effect of Papaverine on the Calcium-dependent K+ Current in Rat Basilar Smooth Muscle Cells. |
| Guang Yi Bai, Jae Woo Cho, Dong Han Han, Tae Ki Yang, Yong Geun Gwak, Chul Jin Kim |
1Department of Neurosurgery, Research Institute of Clinical Medicine and Institute of Cardiovascular Research, Chonbuk National University Medical School and Hospital, Jeonju, Korea. kcj@chonbuk.ac.kr
2Department of Pharmacology, Research Institute of Clinical Medicine and Institute of Cardiovascular Research, Chonbuk National University Medical School and Hospital, Jeonju, Korea. |
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| ABSTRACT |
OBJECTIVE
Papaverine has been used in treating vasospasm following subarachnoid hemorrhage(SAH). However, its action mechanism for cerebral vascular relaxation is not clear.
Potassium channels are closely related to the contraction and relaxation of cerebral smooth muscle. Therefore, to identify the role of potassium and calcium channels in papaverine-induced vascular relaxation, we examine the effect of papaverine on potassium channels in freshly isolated smooth muscle cells from rat basilar artery.
METHODS
The isolation of rat basilar smooth muscle cells was performed by special techniques. The whole cell currents were recorded by whole cell patch clamp technique in freshly isolated smooth muscle cells from rat basilar artery.
Papaverine was added to the bath solution. RESULTS: Papaverine of 100 microM into bath solution increased the amplitude of the outward K+ current which was completely blocked by BKCa(large conductance calcium dependent potassium channels)blocker, IBX(iberiotoxin), and calcium chealator, BAPTA(1, 2-bis(o-aminophenoxy)ethane-N, N, N', N'-tetraacetic acid), in whole cell mode. CONCLUSION: These results strongly suggest that potassium channels may play roles in papaverine-induced vascular relaxation in rat basilar artery. |
| Key Words:
Papaverine; Vasospasm; Potassium channel; Patch-Clamp techniques |
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