The Possible Roles of Matrix Metalloproteinases -1, 2, 3 in Lumbar Disc Herniation.

Jang, Jae Won; Jeong, In Ho; Kim, Soo Han; Lee, Jung Kil; Kim, Jae Hyoo; Lee, Je Hyuk

Original Article

Journal of Korean Neurosurgical Society 2004;36(6): 475-480.

Jae Won Jang, In Ho Jeong, Soo Han Kim, Jung Kil Lee, Jae Hyoo Kim, Je Hyuk Lee

Department of Neurosurgery, Chonnam National University, Medical School, Gwangju, Korea. soohan@chonnam.ac.kr

ABSTRACT

OBJECTIVE
Surgically removed herniated lumbar disc specimen are immunostained to evaluate the production of MMPs(Matrix metalloproteinases) -1, 2, 3 for the investigation of the possible correlation of MMPs in lumbar disc herniation depending on the types of disc herniation. METHODS: The study population consists of 30 patients with lumbar disc herniation. There were 18 men and 12 women with patient age averaging 43.4 years(19-68years). The types of disc herniation identified at the time of surgery were classified as follow ; protruded type(Group 1, 10cases), extruded type(Group 2, 10cases), sequestrated type(Group 3, 10cases). Immunohistochemical study for the MMPs of the herniated disc tissue was performed and results of staining were graded to examine differences in histology among three types of disc herniation . RESULTS: The MMPs immunopositive cells were increased in old patients but statistically it was not significant(p=0.074). A significantly increased incidence of positive cells for MMP-1, 2 was found in the herniated lumbar disc tissue than the control group(p=0.02) but there were no significant differences among the three types of disc herniation. The MMP-3 positive cells were predominantly detected in the sequestrated disc tissue group(p=0.037) more than other groups. CONCLUSION: These results suggest that the MMP-1, 2, 3 may play important roles in the process of degeneration, herniation, and resorption of the lumbar intervertebral discs and that the MMP-3 may express the severity of lumbar disc herniation and play a role in resorption of the sequestrated disc tissue.

Key Words: Lumbar disc herniation; Matrix metalloproteinases; Immunohistochemistry