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Journal of Korean Neurosurgical Society 2003;34(2): 146-152. |
| The Effect of Transglutaminase on the Recovery of Somatosensory Evoked Potentials in the Rat Model of Spinal Cord Injury. |
| Ho Yeon Lee, Byung Duk Kwun, Soo Youl Kim |
1Department of Neurosurgery, Wooridul Spine Hospital, Seoul, Korea. hoyeonle@wooridul.co.kr
2Department of Neurosurgery, Ulsan University College of Medicine, Seoul, Korea.
3Burke Medical Research Institute, Weill Medical College of Cornell University, NY, U.S.A. |
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| ABSTRACT |
OBJECTIVE
The authors present an investigation of the effect of transglutaminase(TG) on the recovery of somatosensory evoked potentials in the rat model of spinal cord injury. METHODS: Female Sprague-Dawley rats(280-310g) were used for this study. Rats were divided into two groups: TG treated and control. The lesion was made by transecting the right dorsal column of the thoracic spinal cord without damage to the vasculature using specially devised micro-glass pipette after laminectomy. For TG treated group, normal saline with TG were injected into the lesion site using micro-pipette through the opening of the dura.
Saltatory repetitive somatosensory evoked potentials(SSEPs) recording were carried out on post-injury 6th and 12th week.
RESULTS: The amplitudes of N19 were 1.28+/-1.60 microV on 6th week, 3.45+/-3.63 microV on 12th week in control group(n=10) and 1.46+/-1.75 microV on 6th week and 5.01+/-2.65 microV on 12th week in TG-treated group(n=11).
Statis-tically significant recovery of SSEPs were seen in TG-treated group(p=0.003, Wilcoxon Signed Ranks Test). In TG-treated group, vacuolated degeneration around the lesion site was rarely observed on histological evaluation.
CONCLUSION
This study demonstrates the possibility of long-term survival and saltatory recording of SSEPs in small animals like rats, after selective spinal cord injury. In addition, this study shows that TG is a factor facilitating the recovery of injured axon of central nervous system. |
| Key Words:
Rat; Somatosensory evoked potentials; Spinal cord injury; Transglutaminase |
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