Polymorphism of Angiotensin Converting Enzyme in Hypertensive Intracerebral Hemorrhage Patients.

Kim, Dong Hwan; Kim, Chul Jin; Kim, Soon Kee; Jho, Jae Woo; Lee, Jung Chung; Kim, Hyoung Ihl; Kho, Kyu Young

Original Article

Journal of Korean Neurosurgical Society 1998;27(4): 425-429.

Dong Hwan Kim, Chul Jin Kim, Soon Kee Kim, Jae Woo Jho, Jung Chung Lee, Hyoung Ihl Kim, Kyu Young Kho

¹Department of Neurosurgery & Physiology, Chonbuk National University, Chonju, Korea.
²School of Medicine, Chonbuk National University, Chonju, Korea.

ABSTRACT

Polymorphism in the angiotensin-converting enzyme(ACE) gene may confer an increased risk of vascular disease. DD type of ACE polymorphism predispose a person to myocardial infarction and IgA nephropathy. The roles of the ACE insertion/deletion polymorphism are unknown in the patients with hypertensive intracerebral hemorrhage (HICH). Our objective is to test and to identify whether genotype distribution of the insertion/deletion polymorphism in ACE gene is different in HICH patients from control subjects. Fifty six HICH patients and sixty one control subjects were studied. Genotypes were determined by the polymerase chain reaction(PCR) with specific oligonucleotide primers flanking the polymorphic region in intron 16 of ACE gene to amplify genomic DNA isolated from patients blood PCR products were separated in 2% agarose gels and bands were visualized by ethidium bromide staining. The PCR reaction amplified a 490bp DNA fragment(II type) from genomic DNA if the subjects had an intact intron 16, and amplified a both 490bp and 190bp DNA fragments(ID type) if subjects had heterozygous polymorphism. While the distribution of ACE polymorphism in control subjects was 26.2%:57.4%:6.4%(II:ID:DD), and the distribution of it in patients with HICH was 37.5%:35.7%:26.8%. Thus, the pattern of distribution was no significant different between control subjects and patients with HICH. The factors of age and sex did not influence on the ratio of distribution in both control and HICH subjects. From these results, we conclude that there is no significant association between I/D polymorphism and HICH.

Key Words: Angiotensin-converting enzymes; Poolymorphism; Gene; Genotyp; Hypertensive intracerebral hemorrhage