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Journal of Korean Neurosurgical Society 1997;26(12): 1644-1652.
The Effect Erythrocyte lysate on Intracellular Calcium Concentration and the Potassium Channel in Cerebral Vasular Smooth Muscle Cells.
Chul Jin Kim
Department of Neurosurgery, Chonbuk National University School of Medicine, Chonju, Korea.
ABSTRACT
Erythrocyte lysate increases intracellular Ca2+[Ca2+]i, causes contraction in cerebral arteries, and is thought to be the causative agent in cerebral vasospasm ; the mechanism of erythrocyte lysate-induced [Ca2+]i mobilization is not, however, clear. This study was undertaken to investigate the action of erythrocyte lysate on [Ca2+]i mobilization by monitoring [Ca2+]i and the Ca2+-dependent K+ channels(Kca) in freshly isolated rat basilar artery smooth muscle cells. In a [Ca2+]i imaging study, erythrocyte lysate produced a biphasic response, a transient peak and prolonged plateau [Ca2+]i elevation ; in the absence of external Ca2+, it induced only a transient peak [Ca2+]i response without a marked plateau phase, indicating that peak [Ca2+]i was due to Ca2+ released from internal stores. The erythrocyte lysate-induced plateau [Ca2+]i response was resistant to nicardipine, a voltage-dependent Ca2+ channel blocker, but was abolished by EGTA. The elevation [Ca2+]i induced by erythrocyte lysate caused contraction in smooth muscle cells. During the electrophysiological study, elevation of [Ca2+]i by erythrocyte lysate increased Kca currents in whole-cell patch-clamp configuration ; this effect was blocked by heparin, an antagonist of IP3 receptors. We conclude that erythrocyte lysate releases Ca2+ from IP3-sensitive intracellular stores and induces Ca2+ entry from voltage-independent Ca2+ pathways.
Key Words: Erythrocyte lysate; K+ channels; IP3; [Ca2+]i; Vasospasm; Cerebral smooth muscle cells
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